Tide Motion Bioreactor CelCradle TM

Tide motion pertains to the oscillation of culture medium into and out of the matrix vessel that intermittently exposes the cells to aeration and nutrition. The upward oscillation exposes the cells to nutrition, while the downward oscillation exposes the cells to aeration and at the same time washes away products and wastes. This gentle oscillation produces no air bubbles and causes very minimal shear stress, which are both detrimental to the cells. VacciXcell offers two types tide motion bioreactors, differing in the production volume capacity.

A Cradle for High Density Cells

CelCradle™ is a disposable bioreactor capable of high-density cell culture for protein expression, virus, and monoclonal antibody production. It is designed based on the concept of bellow-induced intermittent flow of media and air through porous matrices, where cells reside. This provides a low shear, high aeration, and foam-free culture environment.

During operation, the CelCradle™ bottle is partially filled with media and inoculated with cells. The media is raised and lowered alternately to submerge and expose the matrices, creating a dynamic interface between air and media on cell surface to maximize nutrient uptake and oxygen transfer.

CelCradle™ is the only single-use packed bed bioreactor that has linear scalability from bench-scale to production scale with automated cell harvesting.

Features :

• Fully Stainless Steel – SS304L
• Pre-sterilized, ready-to-use, disposable
• Low shear stress, foam-free, no O2 limitation
• Large surface area: one CelCradle™ bottle has a specific surface area of 15,000 cm2, comparable to 20 – 30 roller bottles.
• Compact design: fits in 6ft3 CO2 incubator
• Controlled by Esco Sentinel Gold
• Capacity to produce hundreds of milligrams to several grams of secreted proteins or monoclonal antibodies per month in four bottles
• Few parameters for easy optimization
• Choice of batch, fed-batch, or perfusion culture modes
• Scalability by directly multiplying number of bottles used
• Compatible with most serum-free media
• Specially treated micro carrier surface to grow most anchorage-dependent cells and allow easy harvest of whole cells, cell components or secreted proteins

Applications

• Mammalian and insect cell culture
• Protein and virus production
• Monoclonal antibody production
• Proteome research
• Drug discovery
• Pharmacokinetics study
• Gene and cell therapy